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how to start DFU Bootloader

Hi
I’m trying to start the DFU bootloader so that I can use the DfuSe (v3.0.0) to download software from the PC to the SPI flash on the board, but my pc can’t recognize the Dfu, I have set jumper BOO1: GND, BOOT0: OPEN.
If I download DFU software into the User Flash and execute it the PC recognize it as a DFU.
I’m using a MCBSTM32E board.
Are the any buttons that need to pres to start the DFU, or?
Hope that anyone can give me a hint, thaks.

DFU software is from the “STM32F10xxx USB-FS-Device development kit”